| Family | Scientific Name | Author | Year | Common Name |
| Poeciliidae | Gambusia holbrooki | Girard | 1859 | Eastern Mosquitofish |
Gambusia holbrooki
Unique Characters: Large dusky, black teardrop (often reduced) below eye. One to three rows of spots on dorsal and caudal fins. Dorsal rays usually 7; anal rays 10. Third ray of the anal fin in the male with series of prominent teeth. Often melanistic males present.
Gonopodiums: Gambusia affinis on the left; G. holbrooki on the right. From Rohde et al. (2009), modified from Etnier and Starnes (1993).
Similar Species:
Western Mosquitofish Gambusia affinis. Dorsal rays usually 6; anal rays usually. Third ray of the anal fin of the male without teeth, smooth.
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